An attempt has been made to correlate this information with
9MbAbstractSalmonella FrmR, a member of the RcnR/CsoR family of metalloregulators, has been characterised during the course of this work and was coincidentally confirmed to bind specifically to the frmRA operon, which encodes a putative Zn(II) requiring class III alcohol dehydrogenase. FrmR shares a high degree of similarity with Ni(II)/Co(II) sensing RcnR, in particular conserving two residues of a so called WXYZ motif required to detect metals. Metal binding properties of FrmR were therefore extensively investigated in vitro and its ability steroids steroids, or otherwise, to respond to metals explored in vivo.
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